ÕûÌå¿ÎÌâÍâ°ü·þÎñ
Ò»¡¢ÕûÌå¿ÎÌ⣨ÑϽ÷ÕæÊµ¡¢½á¹û¿É¿¿£©
1. ¸ßͨÁ¿»ùÒòɸѡ£ºÓøßͨÁ¿²âÐò¡¢»ùÒòоƬºÍ CRISPR/Cas9 ÎÄ¿â½øÐйؼü»ùÒòɸѡ¡£
ÕæºËת¼×é²âÐò¡¢ÍâÏÔ×Ó×é²âÐò¡¢»ùÒò±í´ïÆ×оƬ¡¢miRNA ²âÐò/оƬ
m6A ¼×»ù»¯²âÐò¡¢lncRNA ²âÐò/оƬ¡¢µ¥Ï¸°ûת¼×é²âÐò¡¢iTRAQ µ°°×ÖÊ×鶨Á¿
Nat Commun. 2016 Apr 6;7:11215.
2. »ùÒòµ÷¿Ø£ºÓò¡¶¾»òÖÊÁ£ÔØÌå½éµ¼»ùÒò¹ý±í´ï¡¢Çóý»ò¸ÉÈÅ£¬¿ÉÒÔʵÏÖ¶Ô»ùÒò±í´ïµÄÉϵ÷»òϵ÷¡£
¹ý±í´ïÖÊÁ£¡¢¸ÉÈÅÖÊÁ£¡¢CRISPR/Cas9 ÖÊÁ£
ÏÙÏà¹Ø²¡¶¾£¨AAV£©¡¢Âý²¡¶¾£¨LV£©¡¢ÏÙ²¡¶¾£¨ADV£©¡¢Äæ×ªÂ¼²¡¶¾£¨RV£©
3. ϸ°ûˮƽÑéÖ¤£ºÔÚϸ°ûˮƽÑéÖ¤»ùÒòµÄ¹¦ÄÜ£¬°üÀ¨Ï¸°ûÔöÖ³¡¢Ï¸°ûÇ¨ÒÆÓëÇÖÏ®¡¢Ï¸°ûµòÍö¡¢Ï¸°ûÖÜÆÚµÈ¡£
J Hepatol. 2018 Jun;68(6):1214-1227.
4. ¶¯ÎïÌåÄÚʵÑ飺ͨ¹ý¹¹½¨¶¯ÎïÄ£Ðͼì²â»ùÒòˮƽ±ä»¯ºóÒýÆðµÄÖ¸±ê±ä»¯£¬°üÀ¨Æ¤Ï³ÉÁö¡¢Ôλ³ÉÁö¡¢´óСµÈ¡£
J Hepatol. 2018 Jun;68(6):1214-1227.
5. ×÷ÓûúÖÆÑо¿£º½øÒ»²½ÍÚ¾ò³öÓë¹Ø¼ü»ùÒòÏà¹ØµÄÐźÅͨ·»òÆäËü»ùÒò£¬°üÀ¨Ë«Ó©¹âËØÃ¸¼ì²â¡¢RNA pull-down¡¢BioID¡¢CO-IP µÈ¡£
J Hepatol. 2018 Jun;68(6):1214-1227.
µã»÷Á˽â¸ü¶àÏêÇé
¶þ¡¢Éñ¾ÕûÌå¿ÎÌ⣨ÍŶÓרҵ¡¢·þÎñÍêÉÆ£©
1. ¼²²¡Ä£Ð͹¹½¨£º×¨ÒµÌṩÂýÐÔÍ´¡¢ÄÔ×äÖС¢ÒÖÓô¡¢Éñ¾ÍËÐÐÐÔ¼²²¡¡¢ñ²ðïµÈÉñ¾ÏµÍ³¶¯Îï¼²²¡Ä£Ð͵Ĺ¹½¨¡£
Cichon J et al.,Bio Protoc.2018£»Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5673-E5682.
2. »·Â·Ê¾×Ù£º½èÖú²¡¶¾ÔØÌåµÄʾ×Ù¼¼Êõ£¬¿ÉʵÏÖ¶ÔÈ«ÄÔ¡¢ºËÍÅ¡¢»·Â·¡¢Ï¸°û¡¢µ¥Ï¸°û¼°ÑÇϸ°ûÆ÷µÄ±ê¼Ç£¬´Ó¸´ÔÓµÄÉñ¾»·Â·µ½¸ß·Ö±æÂʵĵ¥Éñ¾ÔªÐÎ̬£¬½¨Á¢ÍêÉÆµÄʾ×ÙÌåϵ¡£
Cell Stem Cell. 2020 Mar 5;26(3):331-345.e6.
3. Ôª¼þ²Ù¿Ø£ºÍ¨¹ý²¡¶¾ÔØÌåºÍÄÔ¶¨Î»×¢Éä¼¼Êõ¿ÉÍê³É»ùÒò¹ý±í´ï¡¢»ùÒò³ÁĬ¡¢¹âÒÅ´«Ñ§¡¢»¯Ñ§ÒÅ´«Ñ§µÄ¾«×¼ÌØÒìÐԲٿء£
Science. 2018 Oct 26;362(6413):429-434.
4. ±íÐÍɸѡ¼°±ä»¯£º´Ó·Ö×Óµ½Ï¸°ûÔÙµ½ÐÐΪµÄһվʽ·þÎñ£¬°üÀ¨ WB¡¢qPCR¡¢µçÉúÀí¡¢²¡Àí¡¢ÃâÒß×黯¡¢ÐÐΪѧʵÑé¡£
µã»÷Á˽â¸ü¶àÏêÇé
Èý¡¢m6A ¼×»ù»¯¿ÎÌ⣨˼·Éè¼Æ¡¢·þÎñÍêÉÆ£©
1. ԤʵÑ飺ÕÒµ½Óë RNA ¼×»ù»¯Ïà¹ØµÄøÊÇ·ñÓвîÒì±í´ï»ò¼ì²âÕûÌå m6A ˮƽ£¬Óà qPCR¡¢WB¡¢×é֯оƬ¡¢ÖÊÆ×¼ì²âµÈ£¬ÔÙ½øÐм׻ù»¯Ã¸Çõ͡¢ÇóýºÍ¹ý±í´ïϸ°ûʵÑéºÍ¶¯ÎïʵÑé¡£
Song H et al. Autophagy. 2019£»Chen M. et al. (2018) Hepatology, 67(6): 2254
2. ͨ¹ý m6A-seq ²âÐò£¬¿ÉÒÔÖªµÀÄÄЩ»ùÒòÔÚ RNA ¼×»ù»¯ÐÞÊÎÉÏÓвîÒì,ÔÙ½áºÏת¼×é²âÐò£¬·¢ÏÖ²îÒì»ùÒòµÄ±í´ïˮƽ±ä»¯¡£
Chen M. et al. (2018) Hepatology, 67(6): 2254
3. »ñµÃ RNA ¼×»ù»¯ÐÞÊεÄÄ¿µÄ»ùÒòºó£¬ÔÙ¶Ô m6A ¼×»ù»¯½øÐÐÐÞ¸´£¬¹Û²ìλµãÐÞ¸´Õý³£ºó¶Ôϸ°û±íÐͺÍСÊó³ÉÁö¼°´óСµÄÓ°Ïì¡£
Huang H et al. Nat Cell Biol. 2018
4. ½øÒ»²½ÍÚ¾ò¼×»ù»¯Ã¸Í¨¹ýÄÄЩͨ·¶ÔÄ¿µÄ»ùÒò½øÐе÷¿Ø»òµ°°×»¥×÷¼¼ÊõÍÚ¾òµ°°×£¬°üÀ¨µ°°×Óë DNA »ò RNA »¥×÷¡¢µ°°×Óëµ°°×»¥×÷¡¢°Ð»ùÒòÑéÖ¤µÈ¡£
Chen M. et al. (2018) Hepatology, 67(6): 2254
µã»÷Á˽â¸ü¶àÏêÇé
ËÄ¡¢ÍâÃÚÌåÑо¿¿ÎÌ⣨¾Ñé·á¸»¡¢ÖÊÁ¿¿É¿¿£©
1. ÍâÃÚÌå·ÖÀ룺²îËÙ³¬ËÙÀëÐÄ·¨¿ÉÒÔ»ñµÃ¸ßÖÊÁ¿¡¢¸ß´¿¶ÈµÄÍâÃÚÌ壬Òò´Ë±»ÈÏΪÊÇ·ÖÀë·½·¨Öеġ¸½ð±ê×¼¡¹£¬Ò²ÊǸ߷ÖÎÄÕÂÖг£Ñ¡µÄ·ÖÀë·½·¨¡£
2. ÍâÃÚÌå¼ø¶¨£º³£Óõļø¶¨·½·¨°üÀ¨Í¸Éäµç¾µ£¨TEM£©¡¢ÄÉÃ×Á£¾¶¸ú×Ù·ÖÎö£¨NTA£©¡¢Western Blot£¨WB£©±êÖ¾Îï¼ì²âµÈ¡£
ACS Nano. 2019 Feb 26;13(2):2450-2462.
3. ¸ßͨÁ¿·ÖÎö£ºÍâÃÚÌåÄÚº¬ÓÐÓëϸ°ûÀ´Ô´Ïà¹ØµÄºËËáºÍµ°°×ÖÊ£¬¿ÉÒÔ´«µÝ mRNA¡¢miRNA¡¢lncRNA¡¢circRNA¡¢µ°°×ÖʵȽøÈëÊÜÌåϸ°û£¬²ÎÓëϸ°û¼äͨѶ¡£
Cell Commun Signal. 2019 Aug 16;17(1):96.
4. ÍâÃÚÌåʾ×Ù£º³£ÓÃÇ×Ö¬ÐÔȾÁ϶ÔÍâÃÚÌå½øÐбê¼Ç£¬Ö÷Òª°üÀ¨Á½À࣬һÊÇ PKH67£¨ÂÌÉ«£©ºÍ PKH26£¨ºìÉ«£©£¬¶þÊÇ DiO£¨ÂÌÉ«£©¡¢DiI£¨³ÈÉ«£©ºÍ DiR£¨Éîºì£©¡£
Oncol Rep. 2012 Nov;28(5):1551-8£»2019 Oct 29;19(1):1015.
5. ÌåÄÚÍ⹦ÄÜÑо¿£º½«±ê¼ÇµÄÍâÃÚÌåÓëÊÜÌåϸ°û¹²ÅàÑø£¬¹Û²ìϸ°û¹¦Äܱ仯£¬ÔÙ½«ÍâÃÚÌå×¢ÉäÈ붯ÎïÄ£ÐÍÖУ¬¹Û²ì³ÉÁö´óС£¬²¢¼ì²âÏà¹ØÖ¸±ê¡£
Nat Commun. 2018 Jan 15;9(1):191.